Difference between revisions of "Part:BBa K4463004"

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	Figure1. <i>E.coli</i> expressed SmtA protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.		Figure1. <i>E.coli</i> expressed SmtA protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.
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−		+	We used ICP-MS to measure the concentration of binding Cu(II) and Cd(II) on the surface of <i>E.coli</i>. For detailed information about our measurement method and result analysis, please visit our <a href="https://2022.igem.wiki/xjtlu-china/experiments">Experiments</a>and <a href="https://2022.igem.wiki/xjtlu-china/results">Results</a>on wiki.
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Latest revision as of 09:23, 10 October 2022

CsgA-SmtA
CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA.

IGEM_XJTLU 2022 verified the function of CsgA-SmtA by measuring the concentration of Cu(II) and Cd(II) adsorbed by SmtA protein on the surface of E.coli.

Figure1. E.coli expressed SmtA protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.

Figure1. E.coli expressed SmtA protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

We used ICP-MS to measure the concentration of binding Cu(II) and Cd(II) on the surface of E.coli. For detailed information about our measurement method and result analysis, please visit our Experimentsand Resultson wiki.

Sequence and Features