Difference between revisions of "Part:BBa K4169028"

 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K4169028 short</partinfo>
+
<partinfo>BBa_K4169006</partinfo>
 +
===HepT: a RNase like toxin===
 +
<p>HepT is a toxin that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.</p>
  
−
This is an aerobically initiated promoter that initiates transcription of subsequent sequences under microaerobic and aerobic conditions. The mechanism is based on the fact that Fnr takes over the promoter sequence under anaerobic conditions, competes with RNA polymerase for binding sites, and thus prevents the transcription of subsequent sequences. However, under microaerobic and aerobic conditions, Fnr protein does not bind to the sequence, and thus can continue the subsequent transcription.
 
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
<p>As an RNase enzyme, HepT can degrade mRNA at the transcriptional level, which ultimately leads to cell death
 +
</p>
 +
 +
===Sequence and Features===
 +
<partinfo>BBa_K4169006 SequenceAndFeatures</partinfo>
  
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K4169028 SequenceAndFeatures</partinfo>
 
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
<h3>References</h3>
−
===Functional Parameters===
+
<p>Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli.<i> Nucleic Acids Res.</i> 2015 Jul 13;43(12):6166-79.</p>
−
<partinfo>BBa_K4169028 parameters</partinfo>
+
−
<!-- -->
+

Revision as of 09:23, 10 October 2022


BBa_K4169006

HepT: a RNase like toxin

HepT is a toxin that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.

Usage and Biology

As an RNase enzyme, HepT can degrade mRNA at the transcriptional level, which ultimately leads to cell death

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli. Nucleic Acids Res. 2015 Jul 13;43(12):6166-79.