Difference between revisions of "Part:BBa K4137006:Design"
Line 12: Line 12:
  
  
−
[[File:ccda-tag-final-benchling-linear.png|800px|thumb|center|Fig.1 Benching linear map of ccdA final construct.]]
 
  
  

Revision as of 07:53, 10 October 2022

CcdA expressing construct



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

We chose to use p_mles, the mleR regulatory site as mleR can be induced by malate acid which surrounds the tree roots. CcdA is the antitoxin of the toxin Ccdb which kills chi18h8 when the probiotic isn’t near the roots of the banana tree. The 6xHis is used for protein quantification and is the most commonly used purification his-tag.





Source

https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://parts.igem.org/Part:BBa_K1659003


References