Difference between revisions of "Part:BBa K4137013:Design"

 
 
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The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations. The 6xHis tag is used for protein quantification and is the most commonly used purification his-tag.
 
The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations. The 6xHis tag is used for protein quantification and is the most commonly used purification his-tag.
  
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[[File:ccdb-tag-linear.png|800px|thumb|center|Fig.1 Benching linear map of final ccdB construct.]]
  
  

Latest revision as of 07:52, 10 October 2022


ccdB toxin secretion pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 289


Design Notes

pLac promoter is chosen for constitutive expression of ccdB. The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations. The 6xHis tag is used for protein quantification and is the most commonly used purification his-tag.

Fig.1 Benching linear map of final ccdB construct.


Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1 https://sci-hub.hkvisa.net/10.1016/0022-2836(85)90070-1 https://2018.igem.org/Team:Pasteur_Paris/Kill


References