Difference between revisions of "Part:BBa K4194021"

 
 
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E. coli fatty acid transporter
 
E. coli fatty acid transporter
  
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===Usage and Biology===
 
  
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===Usage and Biology===
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The permeability of fatty acids across the cell membrane is very low because the outer layer of the outer membrane of Escherichia coli consists mainly of lipopolysaccharides and is the main barrier for the transport of hydrophobic compounds to Escherichia coli, so the bioconversion of fatty acids within the cell may be slow due to supply constraints. The expression level of fatty acid transporter fadL has a significant effect on the biotransformation of E. coli. It is an essential carrier for the transport of exogenous fatty acids, and its presence facilitates the transport of fatty acids across the outer membrane, especially those containing 10-18 C.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4194021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4194021 SequenceAndFeatures</partinfo>
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===Experiment and result===
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BUCT-China overexpressed fadL gene in Escherichia coli, and verified by comparing the product yield obtained by overexpressing fadL and not overexpressing fadL gene. The results showed that the yield of polyhydroxyfatty acid lipid (PHFA) increased by nearly 1.7 times in engineering bacteria overexpressing fadL. These results indicate that the transporter protein expressed by fadL has a significant effect on the yield of PHFA.
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[[File:FadL.png|700px|thumb|left|The transporter protein expressed by fadL has a significant effect on PHFA yield]]
  
  

Latest revision as of 06:12, 10 October 2022


fadL

E. coli fatty acid transporter


Usage and Biology

The permeability of fatty acids across the cell membrane is very low because the outer layer of the outer membrane of Escherichia coli consists mainly of lipopolysaccharides and is the main barrier for the transport of hydrophobic compounds to Escherichia coli, so the bioconversion of fatty acids within the cell may be slow due to supply constraints. The expression level of fatty acid transporter fadL has a significant effect on the biotransformation of E. coli. It is an essential carrier for the transport of exogenous fatty acids, and its presence facilitates the transport of fatty acids across the outer membrane, especially those containing 10-18 C. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1108
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiment and result

BUCT-China overexpressed fadL gene in Escherichia coli, and verified by comparing the product yield obtained by overexpressing fadL and not overexpressing fadL gene. The results showed that the yield of polyhydroxyfatty acid lipid (PHFA) increased by nearly 1.7 times in engineering bacteria overexpressing fadL. These results indicate that the transporter protein expressed by fadL has a significant effect on the yield of PHFA.

The transporter protein expressed by fadL has a significant effect on PHFA yield