Difference between revisions of "Part:BBa K4147009"

Latest revision as of 06:09, 10 October 2022

MsrA2 antimicrobial protein for purification with 6X-His tag

Antimicrobial protein from the arboreal tree frog Phyllomedusa bicolor, dermaseptin-b1 gene (AKA MsrA2) (BBa_K2577001) fused with a 6X-Hist tag for purification by metal affinity chromatography.

Usage and Biology

Dermaseptins (DRSs) are peptides produced by Hylid frogs, a group of ɑ-helical shaped polycationic and short peptides (21-34 residues) containing a highly preserved tryptophan residue on N-terminal 3rd position, with hydrophobic residues and the polar cationic residues clusters in opposite sides [1]. The majority of the dermaseptin family peptides exhibit a wide variety of antibacterial activities. A broad range of microorganisms, including mollicutes, bacteria, fungi, protozoa, yeast, and enveloped viruses are all susceptible to the dermaseptins. Despite having a very similar sequence, the dermaseptins vary in how well they can neutralize different substances [2]. Dermaseptins also exhibit efficacy against various kinds of human cancer, making them anticancer peptides in addition to their antimicrobial capabilities.[1].

Sequence and Features

REFERENCES

[1] Bartels J. et al. Dermaseptins, Multifunctional Antimicrobial Peptides: A Review of Their Pharmacology, Effectivity, Mechanism of Action, and Possible Future Directions.

[2] Nicolas, P., & el Amri, C. (2009). The dermaseptin superfamily: A gene-based combinatorial library of antimicrobial peptides. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1788(8), 1537–1550.