Difference between revisions of "Part:BBa K4137007:Design"
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The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations.
 
The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations.
  
 +
[[File:ccdb-tag-linear.png|800px|thumb|center|Fig.1 Benching linear map of final ccdB construct.]]
  
  

Revision as of 04:41, 10 October 2022


pLac+RBS+CcdB-Myc+B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 271


Design Notes

RBS is used to enhance ccdB synthesis. pLac promoter is chosen for constitutive expression of ccdB. The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations.

Fig.1 Benching linear map of final ccdB construct.


Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1 https://sci-hub.hkvisa.net/10.1016/0022-2836(85)90070-1 https://2018.igem.org/Team:Pasteur_Paris/Kill

References