Difference between revisions of "Part:BBa K4147010"

 
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<partinfo>BBa_K4147010 SequenceAndFeatures</partinfo>
 
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===Characterization of MCherry RFP Construct.png===
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<center>[[File:MCherry-Tec-Chihuahua.png |300px]]</center>
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<center><b>Figure 1</b>. SnapGene®️ map of BioBrick BBa_K4147008.</center>
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4147010 parameters</partinfo>
 
<partinfo>BBa_K4147010 parameters</partinfo>
 
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Revision as of 23:25, 9 October 2022


mCherry (RFP) construct with LacI regulated promoter for bacterial expression

This mCherry reporter is one of several "second-generation" monomeric fluorescent proteins. The proposed construct for its expression it's conformed by a LacI regulated promoter (BBa_R0010), an RBS (BBa_B0032) and a double terminator (BBa_B0015). Lastly this construct was optimized for its production on E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization of MCherry RFP Construct.png

MCherry-Tec-Chihuahua.png
Figure 1. SnapGene®️ map of BioBrick BBa_K4147008.