Difference between revisions of "Part:BBa K3748011"
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<partinfo>BBa_K3748011 parameters</partinfo>
 
<partinfo>BBa_K3748011 parameters</partinfo>
 
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==Team Estonia_TUIT characterization of BBa_K3748015 (<i>pPAB1</i>)==
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Constitutive <i>pPAB1</i> promoter (Michael E. Lee et. al, 2015), controls a gene encoding the <i>Pab1<i> protein. This protein binds Poly(A) and is involved in the control of poly(A) tail length on nuclear mRNA transcripts (N Amrani et. al, 1997).
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<i>pPAB1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pPAB1</i> showed a 19-fold increase in fluorescence intensity (figure 1).
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[[Image:PPAB1 Estonia TUIT.png|600px|thumb|center|'''Figure 1:''' <b>Graph depicting median fluorescence intensity of <i>pPAB1</i> and control strain</b>]]
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<b>References:</b>
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<ul>
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<li>A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. Michael E. Lee, William C. DeLoach, Bernardo Cervantes, and John E. Dueber, 2015</li> 
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<li>Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro, N Amrani, M Minet, M Le Gouar, F Lacroute, F Wyers, 1997</li>
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</ul>

Revision as of 22:13, 9 October 2022


pPAB1

S.cerevisiae medium strenght promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Team Estonia_TUIT characterization of BBa_K3748015 (pPAB1)

Constitutive pPAB1 promoter (Michael E. Lee et. al, 2015), controls a gene encoding the Pab1<i> protein. This protein binds Poly(A) and is involved in the control of poly(A) tail length on nuclear mRNA transcripts (N Amrani et. al, 1997).

<i>pPAB1 promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, pPAB1 showed a 19-fold increase in fluorescence intensity (figure 1).

Figure 1: Graph depicting median fluorescence intensity of pPAB1 and control strain

References:

  • A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. Michael E. Lee, William C. DeLoach, Bernardo Cervantes, and John E. Dueber, 2015
  • Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro, N Amrani, M Minet, M Le Gouar, F Lacroute, F Wyers, 1997