Difference between revisions of "Part:BBa K4321004"

Latest revision as of 21:04, 9 October 2022

mScarlet

mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a synthetic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.

Usage and Biology

This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and emission maxima of 594 nanometers.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 512
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074

mScarlet . SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=mScarlet

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4321004 short</partinfo>
-mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a syntheic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.
+mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a synthetic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.
 ===Usage and Biology===
-This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and and emission maxima of 594 nanometers.
+This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and emission maxima of 594 nanometers.
 ===Sequence and Features===