Difference between revisions of "Part:BBa K3748015"

(→‎Team Estonia_TUIT characterization of BBa_K3748015 (pREV1))
(→‎Team Estonia_TUIT characterization of BBa_K3748015 (pREV1))
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<i>pREV1</i> is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of <i>Rev1</i>, a gene encoding bi-functional  DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA  repair (Lawrence CW, 2004).
 
<i>pREV1</i> is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of <i>Rev1</i>, a gene encoding bi-functional  DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA  repair (Lawrence CW, 2004).
  
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<i>pREV1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pREV1</i> demonstrated a five-fold increase in fluorescence intensity, respectively (figure 1). The results confirm the data from Michael E. Lee’s article from 2015, where the <i>pREV1</i> was the weakest promoter present in the kit.
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<i>pREV1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pREV1</i> demonstrated a five-fold increase in fluorescence intensity (figure 1).
  
  

Revision as of 20:51, 9 October 2022


pREV1

Weak strenght yeast promoter S.cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 705
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Team Estonia_TUIT characterization of BBa_K3748015 (pREV1)

pREV1 is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of Rev1, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence CW, 2004).

pREV1 promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, pREV1 demonstrated a five-fold increase in fluorescence intensity (figure 1).


Figure 1: Graph depicting median fluorescence intensity of pREV1 and control strain

References:

  • A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. Michael E. Lee, William C. DeLoach, Bernardo Cervantes, and John E. Dueber, 2015
  • Cellular functions of DNA polymerase zeta and Rev1 protein, Lawrence CW (2004), Adv Protein Chem