Difference between revisions of "Part:BBa K4147013"
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<partinfo>BBa_K4147013 short</partinfo>
 
<partinfo>BBa_K4147013 short</partinfo>
  
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Lower part of the cassette for production of siRNA. It is cmpossed of  a reverse T7 promoter with a RBS (BBa_K4147014) and a T7 terminator (BBa_K864600).  
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Lower part of the cassette for production of siRNA. It is compossed of  a reverse T7 promoter with a RBS (BBa_K4147014) and a T7 terminator (BBa_K864600).  
  
 
In order to produce the siRNAs, this part is assembled with the upper part.
 
In order to produce the siRNAs, this part is assembled with the upper part.

Revision as of 19:43, 9 October 2022


Down part of cassette for siRNA production

Lower part of the cassette for production of siRNA. It is compossed of a reverse T7 promoter with a RBS (BBa_K4147014) and a T7 terminator (BBa_K864600).

In order to produce the siRNAs, this part is assembled with the upper part.

Usage and Biology

To produce the double-stranded siRNAs, an expression system has been designed and must be assembled. The assembly method is based on the one shown by the iGEM Ecuador 2021 team.

The figure below shows the assembly strategy that is planned to be used.

action.
Assembly strategy for siRNA production

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]