Difference between revisions of "Part:BBa K4147012"
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<partinfo>BBa_K4147012 short</partinfo> | <partinfo>BBa_K4147012 short</partinfo> | ||
| − | Upper part of the cassette for siRNA expression. This part is part of the construct expressing the siRNA flanking the mRNA of the RXLR gene of <i>P. capsici</i>. | + | Upper part of the cassette for siRNA expression. This part is part of the construct expressing the siRNA flanking the mRNA of the RXLR gene of <i>P. capsici</i>. It is cmpossed of a reverse T7 terminator with a RBS (BBa_K4147015) and a T7 promotor and RBS (BBa_K864600). |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:36, 9 October 2022
Up part of cassette for siRNA production of RXLR gene for P. capsici
Upper part of the cassette for siRNA expression. This part is part of the construct expressing the siRNA flanking the mRNA of the RXLR gene of P. capsici. It is cmpossed of a reverse T7 terminator with a RBS (BBa_K4147015) and a T7 promotor and RBS (BBa_K864600).
Usage and Biology
To produce the double-stranded siRNAs, an expression system has been designed and must be assembled. The assembly method is based on the one shown by the iGEM Ecuador 2021 team.
The figure below shows the assembly strategy that is planned to be used.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]