Difference between revisions of "Part:BBa K4147014"
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===Usage and Biology===
 
===Usage and Biology===
  
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To produce the double-stranded siRNAs, an expression system has been designed and must be assembled.
 
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The assembly method is based on the one shown by the <b>iGEM Ecuador 2021 team</b>.
 
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The figure below shows the assembly strategy that is planned to be used.
 
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[[File:Up_RXLR.png|600px|thumb|center|alt=action.|Assembly strategy for siRNA production]]
 
  
 
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Revision as of 19:11, 9 October 2022


Reverse of BBa_K525998.

This part is used for the expression of siRNA. It consists in the reves¿rse sequence of a T7 promoter and a RBS.

In order to produce double-stranded siRNAs, we need the sequences in both directions. This is why we designed the reverse sequence for this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]