Difference between revisions of "Part:BBa K4428001:Design"

 
(Design)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Designed such that only the metal binding domain of the protein is obtained
+
Codon optimised for the chassis E. coli by changing the 150<sup>th</sup> nucleotide from T to C
  
  

Revision as of 18:42, 9 October 2022


PbrR MBD - Lead-binding domain of PbrR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 20


Design Notes

Codon optimised for the chassis E. coli by changing the 150th nucleotide from T to C


Source

Truncated part of PbrR

References