Difference between revisions of "Part:BBa J428061"

Revision as of 17:30, 9 October 2022

Cas_variants_hLwCas13a

Cas_variants_hLwCas13a ,synthesised from Leptotrichia wadeii

Information contributed by City of London UK (2022)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

• Group: City of London UK 2022
• Author: Julian Chen
• Summary: Added information collated from existing scientific studies

Usage and Biology

Cas13 is a programmable endonuclease that targets RNA, as opposed to DNA, used in Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems. It is activated by a ssRNA sequence complementary to its cRNA spacer, which then induces non-specific RNase activity that cleaves all nearby RNA sequences. It may be used for efficient and specific RNA knockdown and RNA sequencing in mammalian cells. ^[1]

Cas13a was considered for use by the City of London iGEM 2022 Team, alongside Cas12a, but was ultimately dropped due to the need to cleave amplified DNA sequences.

It utilises a PAM: 3’-H

Sequence and Features

1. ↑ Pawluk, A., n.d. CRISPR Systems: What’s the Difference?. [online] Cell.com. Available at: <https://www.cell.com/pb-assets/products/research-arc/infographics/CrisprVizInfo_vol1a-1539275242767.pdf> [Accessed 9 October 2022].