Difference between revisions of "Part:BBa K4192132"

Revision as of 17:27, 9 October 2022

Plac-RBS-yf1(N37C)-RBS-fixJ-PfixK2-RBS-mcherry

The gene yf1, fixJ and PfixK2 make up the system of blue light regulation. Under dark conditions, phosphate groups are transferred from Yf1 protein to FixJ protein, and the phosphorylated FixJ protein activates the PfixK2 promoter, which in turn activates downstream gene expression. Under light induction, the phosphorylation of Fixj protein was blocked and the expression of genes regulated by the PfixK2 promoter was suppressed.The mCherry protein emits red fluorescence to characterize the efficiency of control elements in regulating gene expression. We mutated position 37 N of YF1 to C to improve its response to blue light and better regulate gene expression.

Usage

Light control system can regulate gene expression by light intensity. MCherry has an excitation wavelength of 580nm and an emission wavelength of 610nm. Quantitative detection of mCherry can be achieved by detecting the optical signal at 610nm. This composition part examines the ability of the light control system containing the yf1 mutation to control gene expression and compares it with the normal light control system (BBa_ K4192130). The mutant yf1 (N37C) to expand the dynamic range of regulated gene expression under different signal intensities of the light control system, which would be more conducive to the abundant expression of downstream genes under dark conditions.

Biology

Sequence and Features