Difference between revisions of "Part:BBa K1724002"

Revision as of 17:12, 9 October 2022

MerR

The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.

The quantification data of MerR/CadA operon can be seen in BBa_K1724000.[[1]]

IMPROVEMENT: iGEM Peshawar 2017 has gotten this part synthesized and has submitted it to the registry for future use by teams.

Bioinformatic characterization – iGEM Edinburgh-UHAS_Ghana 2022

We characterized the structure and metal interaction of this part. We compared the metal binding properties of this mutated form of MerR (henceforth referred to as mut_MerR) to wild-type MerR, to see if the affinity for the heavy metal ion remains constant. We also generated a novel structure for mut_MerR, as well as a structure bound to pmerT, to show it can theoretically function in vivo.

test

Sequence and Features