Difference between revisions of "Part:BBa K4414001"
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===Functional characterization ===
 
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We can connect NLS to EGFP and observe the location of fluorescent proteins to verify the function of NLS.
 
  
 
===Reference===
 
===Reference===
 
[1].Cokol, M., Nair, R., & Rost, B. (2000). Finding nuclear localization signals. EMBO Reports, 1(5), 411–415. https://doi.org/10.1093/embo-reports/kvd092
 
[1].Cokol, M., Nair, R., & Rost, B. (2000). Finding nuclear localization signals. EMBO Reports, 1(5), 411–415. https://doi.org/10.1093/embo-reports/kvd092

Revision as of 16:58, 9 October 2022


nuclear localization signal（NLS）

This nuclear localization sequence（NLS） derived from SV40 directs the protein into the nucleus.

Usage and Biology

A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus.[1]

Sequecing

The plasmid was sequenced correct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference

[1].Cokol, M., Nair, R., & Rost, B. (2000). Finding nuclear localization signals. EMBO Reports, 1(5), 411–415. https://doi.org/10.1093/embo-reports/kvd092