Difference between revisions of "Part:BBa K4241011"
 
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This is a scar for enhanced T7 promoter in between T7 promoter and RBS.  The sequence is derived from the following publication: https://www.nature.com/articles/s42003-020-0939-8
 
This is a scar for enhanced T7 promoter in between T7 promoter and RBS.  The sequence is derived from the following publication: https://www.nature.com/articles/s42003-020-0939-8
  
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===Usage and Biology===
 
  
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===References===
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Shilling, P. J., Mirzadeh, K., Cumming, A. J., Widesheim, M., Köck, Z., &amp; Daley, D. O. (2020). Improved designs for pet expression plasmids increase protein production yield in escherichia coli. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-0939-8
  
  

Latest revision as of 16:55, 9 October 2022


Scar for pMET-T7-RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Overview

This is a scar for enhanced T7 promoter in between T7 promoter and RBS. The sequence is derived from the following publication: https://www.nature.com/articles/s42003-020-0939-8


References

Shilling, P. J., Mirzadeh, K., Cumming, A. J., Widesheim, M., Köck, Z., & Daley, D. O. (2020). Improved designs for pet expression plasmids increase protein production yield in escherichia coli. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-0939-8