Difference between revisions of "Part:BBa K4241025"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Ulp1 is an enzyme designed to cleave the SUMO-tag from it's fusion protein. This reaction is specific allowing for the specific and induced cleavage of SUMO. | Ulp1 is an enzyme designed to cleave the SUMO-tag from it's fusion protein. This reaction is specific allowing for the specific and induced cleavage of SUMO. | ||
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This is the coding sequence for Ulp1 to be used in bacterial protein expression systems | This is the coding sequence for Ulp1 to be used in bacterial protein expression systems | ||
Latest revision as of 16:37, 9 October 2022
Ulp1 N-terminal (SUMO protease)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Overview
This part is the Ulp1 enzyme used to cleave the fusion partner at SUMO c-terminal without a scar. This is derived from iGEM distribution kit 2022, plate 2.
Usage and Biology
Ulp1 is an enzyme designed to cleave the SUMO-tag from it's fusion protein. This reaction is specific allowing for the specific and induced cleavage of SUMO.
This is the coding sequence for Ulp1 to be used in bacterial protein expression systems