Difference between revisions of "Part:BBa K200009"
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This sequence codes for the restriction enzyme DpnII.<br> | This sequence codes for the restriction enzyme DpnII.<br> | ||
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===Detail=== | ===Detail=== | ||
This is a Type II restriction enzyme that recognises the sequence GATC. Its activity can be blocked by dam methylation. | This is a Type II restriction enzyme that recognises the sequence GATC. Its activity can be blocked by dam methylation. | ||
Revision as of 13:36, 29 September 2009
DpnII Composite
This sequence codes for the restriction enzyme DpnII.
Detail
This is a Type II restriction enzyme that recognises the sequence GATC. Its activity can be blocked by dam methylation.
The working temperature is 37°C.
As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.
Usage and Biology
DpnII is a 4 base cutter, leaving a 4 base overhang.
The cutting site is as shown:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[http://www.neb.com/nebecomm/products/productR0543.asp Information from NEB]