Difference between revisions of "Part:BBa K4241025"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4241025 short</partinfo>
 
<partinfo>BBa_K4241025 short</partinfo>
 +
<partinfo>BBa_K4241025 SequenceAndFeatures</partinfo>
  
−
This is Ulp1 protein, which could be cleave fusion partner of SUMO c-terminal without scar. This is from the distribution kit plate 2 of 2022 of Ulp1.  
+
===Overview===
 +
This part is the Ulp1 enzyme used to cleave the fusion partner at SUMO c-terminal without a scar. This is derived from iGEM distribution kit 2022, plate 2.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
Ulp1 is an enzyme designed to cleave the SUMO-tag from it's fusion protein. This reaction is specific allowing for the specific and induced cleavage of SUMO.
 +
<br/?>
 +
This is the coding sequence for Ulp1 to be used in bacterial protein expression systems
 +
 +
  
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K4241025 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 16:32, 9 October 2022


Ulp1 N-terminal (SUMO protease)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Overview

This part is the Ulp1 enzyme used to cleave the fusion partner at SUMO c-terminal without a scar. This is derived from iGEM distribution kit 2022, plate 2.

Usage and Biology

Ulp1 is an enzyme designed to cleave the SUMO-tag from it's fusion protein. This reaction is specific allowing for the specific and induced cleavage of SUMO.
This is the coding sequence for Ulp1 to be used in bacterial protein expression systems