Difference between revisions of "Part:BBa K200010"

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This sequence codes for the restriction enzyme TaqI.<br>
 
This sequence codes for the restriction enzyme TaqI.<br>
  
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===Detail===
 
This is a Type II restriction enzyme that recognises the sequence TCGA.  Its activity can be blocked by dam methylation. <br>
 
This is a Type II restriction enzyme that recognises the sequence TCGA.  Its activity can be blocked by dam methylation. <br>
  

Revision as of 13:19, 29 September 2009

TaqI Composite

This sequence codes for the restriction enzyme TaqI.


Detail

This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation.

The working temperature is 65°C.

As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.


Usage and Biology

The restriction enzyme TaqI, is isolated from the bacterium Thermus aquaticus. 1

It cuts at a site that is near to where early and late messenger RNA transcription starts in opposite directions. Furthermore, it is a 4 base cutter, leaving a 2 base overhang.

The cutting site is as shown:

II09 TaqI.png 1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 36
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175

References

<biblio>

  1. 1 pmid=204628

</biblio>

2. [http://www.neb.com/nebecomm/products/productM0219.asp Information from NEB]