Difference between revisions of "Part:BBa K4192130"

Revision as of 16:08, 9 October 2022

Plac-RBS-yf1-RBS-fixJ-Pfixk2-RBS-mcherry

The gene yf1, fixJ and PfixK2 make up the system of blue light regulation. Under dark conditions, phosphate groups are transferred from Yf1 protein to FixJ protein, and the phosphorylated FixJ protein activates the PfixK2 promoter, which in turn activates downstream gene expression. Under light induction, the phosphorylation of Fixj protein was blocked and the expression of genes regulated by the PfixK2 promoter was suppressed.The mCherry protein emits red fluorescence to characterize the efficiency of control elements in regulating gene expression.

Usage

Light control system can regulate gene expression by light intensity. mCherry has an excitation wavelength of 580nm and an emission wavelength of 610nm. Quantitative detection of mcherry can be achieved by detecting the optical signal at 610nm. This composite part was constructed to test the ability of the original light control system to regulate gene expression compared with the light control system containing yf1 mutation(BBa_K4192132).

Biology

Fig.2 Regulatory mechanism of YF1 construction^{[1]</b> Sequence and Features}