Difference between revisions of "Part:BBa K200007"

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As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise cellulase to a tunable threshold. Following that, E.coli is to be self-encapsulated so as to protect cellulase through the stomach, until its release in the small intestine. Cellulase will then be able to digest the cellulose found in our food.
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<br><br>The gene was used as part of the Imperial iGEM 2009 <i>The E.ncapsulator</i> project as one of the showcase proteins of the protein production mechanism of the project.  
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:15, 29 September 2009

Cellulase

Overview

This is the coding sequence for [http://en.wikipedia.org/wiki/Cellulase cellulase] production in E.coli. It codes for endo-b-1,4-glucanase E (CelE) cellulase, which is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.1

More Details

Cellulase mainly catalyses the reactions that changes crystalline cellulose to cellobiose2 and then finally to glucose. It also catalyses, to a small extent, the break down of carboxymethyl cellulose. This cellulase is protease resistant.




The gene was used as part of the Imperial iGEM 2009 The E.ncapsulator project as one of the showcase proteins of the protein production mechanism of the project.


Usage and Biology

It can be used in gram positive bacteria.

The enzyme has a C-end cellulosome-binding domain (CBD), which is used to deliver its resident catalytic domain to the cellulosome. The cellulosome is multi-subunit complex that is involved in the hydrolysis of crystalline cellulose. [http://www.springerlink.com/content/lp8g657n13g76187/fulltext.pdf [3]]

In Clostridum, CelE activity activates other cellulosomal enzymes synergistically.2


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 700
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI] <biblio>

  1. 1 pmid=9055408
  2. 2 pmid=10714996

</biblio>