Difference between revisions of "Part:BBa K4129001"
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<partinfo>BBa_K4129001 short</partinfo> | <partinfo>BBa_K4129001 short</partinfo> | ||
| − | The part consists of the 1000 bp upstream of Formate Dehydrogenase 2 (FDH2) CDS from Saccharomyces cerevisiae. FDH2 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified from gDNA through PCR and used to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression | + | The part consists of the 1000 bp upstream of Formate Dehydrogenase 2 (FDH2) CDS from Saccharomyces cerevisiae. FDH2 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified from gDNA through PCR and used to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 15:12, 9 October 2022
Promoter of the Saccharomyces cerevisiae FDH2 gene
The part consists of the 1000 bp upstream of Formate Dehydrogenase 2 (FDH2) CDS from Saccharomyces cerevisiae. FDH2 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified from gDNA through PCR and used to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 275
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]