Difference between revisions of "Part:BBa K4286100"
| Line 11: | Line 11: | ||
===modeling=== | ===modeling=== | ||
| + | ===Sequencing=== | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:36, 9 October 2022
Effector device in timed suicide switch
2022 SZU-China plans to design a timed suicide switch mediated by a classical synthetic gene oscillator. The core of the effector is the MazEF toxin-antitoxin system, in which the antitoxin mazE is controlled by the Promoter tetR , and the antitoxin mazF is controlled by the constitutive promoter PJ23110. The MazEF system is an important component of the subtilisin-antitoxin system, which leads to programmed cell death. MazF is an mRNA endonuclease that specifically cleaves a five-base UACAU sequence on RNA. In the presence of MazE, MazF has a higher affinity for MazE than its RNA substrate. MazE binds to MazF to occupy its active site, rendering it unable to bind or cleave the mRNA.
Usage and Biology
Assembly
modeling
Sequencing
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 481
Illegal NheI site found at 504 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 582
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]