Difference between revisions of "Part:BBa K239000:Design"

(→‎Design Notes)
(→‎References)
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===References===
 
===References===
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Genome-Wide Analyses of Escherichia coli Gene Expression Responsive to the BaeSR Two-Component Regulatory System
 
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Kunihiko Nishino, Takeshi Honda, and Akihito Yamaguchi
 
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J Bacteriol. 2005 March; 187(5): 1763–1772. doi: 10.1128/JB.187.5.1763-1772.2005.
 
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PMCID: PMC1063996
 
  
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Signal Detection and Target Gene Induction by the CpxRA Two-Component System
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Patricia A. DiGiuseppe and Thomas J. Silhavy
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J Bacteriol. 2003 April; 185(8): 2432–2440. doi: 10.1128/JB.185.8.2432-2440.2003.
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PMCID: PMC152615
  
  

Revision as of 16:27, 28 September 2009

DegP promoter, activated by Phosporylated CpxR and SigmaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. Only one SpeI restriction site had to be removed. The sequence starts 3 bp before the first CpxR binding site.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

References

Signal Detection and Target Gene Induction by the CpxRA Two-Component System Patricia A. DiGiuseppe and Thomas J. Silhavy J Bacteriol. 2003 April; 185(8): 2432–2440. doi: 10.1128/JB.185.8.2432-2440.2003. PMCID: PMC152615


Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli Stéphanie Bury-Moné, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, Sandrine Imbeaud, Annick Jacq, and Philippe Bouloc PLoS Genet. 2009 September; 5(9): e1000651. Published online 2009 September 18. doi: 10.1371/journal.pgen.1000651. PMCID: PMC2731931