Difference between revisions of "Part:BBa K239001:Design"

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Zoetendal EG, Smith AH, Sundset MA, Mackie RI. 2008
 
Zoetendal EG, Smith AH, Sundset MA, Mackie RI. 2008
 
Appl Environ Microbiol. 2008 Jan;74(2):535-9. Epub 2007 Nov 26.
 
Appl Environ Microbiol. 2008 Jan;74(2):535-9. Epub 2007 Nov 26.
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 +
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Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli
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Stéphanie Bury-Moné, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, Sandrine Imbeaud, Annick Jacq, and Philippe Bouloc
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PLoS Genet. 2009 September; 5(9): e1000651. Published online 2009 September 18. doi: 10.1371/journal.pgen.1000651.
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PMCID: PMC2731931

Revision as of 16:21, 28 September 2009

Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

References

The BaeSR two-component regulatory system mediates resistance to condensed tannins in Escherichia coli. Zoetendal EG, Smith AH, Sundset MA, Mackie RI. 2008 Appl Environ Microbiol. 2008 Jan;74(2):535-9. Epub 2007 Nov 26.


Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli Stéphanie Bury-Moné, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, Sandrine Imbeaud, Annick Jacq, and Philippe Bouloc PLoS Genet. 2009 September; 5(9): e1000651. Published online 2009 September 18. doi: 10.1371/journal.pgen.1000651. PMCID: PMC2731931