Difference between revisions of "Part:BBa K4165053"
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_K4165063), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_K4165063), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721). | This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_K4165063), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_K4165063), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part performs a multifunction role in our system, the Plug-Sink system. The H1A (BBa_K4165000) peptide directs the whole peptide to bind to the PDZ HtrA1 protein (BBa_K4165004). The second part is the binding peptide which directs the whole peptide-HtrA1 complex to the tau (BBa_K4165009) and amyloid (BBa_K4165005) aggregates. The last part makes the whole system switch on or off based on the presence of the target protein, thus part is the WAP inhibitor (BBa_K4165008). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K4165051 SequenceAndFeatures</partinfo> |
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| − | === | + | ===Dry-Lab characterization=== |
| − | < | + | |
| − | < | + | |
| + | ===Modelling=== | ||
| + | PDB Structure: | ||
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| + | <html> | ||
| + | <p><img src="https://static.igem.wiki/teams/4165/wiki/33.png" style="margin-left:200px;" alt="" width="500" /></p> | ||
| + | </html> | ||
| + | |||
| + | Figure 1. The 3D structure of switch 33 modelled by TRrosetta. | ||
Revision as of 14:11, 9 October 2022
Status: 500
Content-type: text/html
Software error:
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_K4165063), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_K4165063), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721).
Usage and Biology
This part performs a multifunction role in our system, the Plug-Sink system. The H1A (BBa_K4165000) peptide directs the whole peptide to bind to the PDZ HtrA1 protein (BBa_K4165004). The second part is the binding peptide which directs the whole peptide-HtrA1 complex to the tau (BBa_K4165009) and amyloid (BBa_K4165005) aggregates. The last part makes the whole system switch on or off based on the presence of the target protein, thus part is the WAP inhibitor (BBa_K4165008).
Sequence and Features Status: 500 Content-type: text/html
Software error:
Can't find string terminator '"' anywhere before EOF at /websites/parts.igem.org/cgi/lib/Range.pm line 311. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Dry-Lab characterization
Modelling
PDB Structure:
Figure 1. The 3D structure of switch 33 modelled by TRrosetta.