Difference between revisions of "Part:BBa K4389005"
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L1 protein from Vaccinia firus | L1 protein from Vaccinia firus | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | A27 is a 110 amino acids protein, that consist of the heparin-binding domain, a coiled-coil domain, and the A17 binding leucine zipper domain. The heparin-binding domain contains 21-34 amino acids and has the core sequence KKPE, which is 26-29 amino acids. KKPE is essential for heparin sulfate attachment. Coiled-coil domain, 43-84 amino acids, contributes to self-oligomerization in vitro. Two cysteines at 71 and 72 create a disulfide bond for the self-assembly of A27, and it interacts with A26. The crystal structure of A27 is a trimeric assembly, consisting of three α-helices, two parallel and one antiparallel. Trimerization can be observed in the N-terminal section. Interaction between trimers occurs at the C-terminal region | ||
| + | <!-- Add more about the biology of this part here | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:05, 9 October 2022
L1 protein encoding sequence
L1 protein from Vaccinia firus
Usage and Biology
A27 is a 110 amino acids protein, that consist of the heparin-binding domain, a coiled-coil domain, and the A17 binding leucine zipper domain. The heparin-binding domain contains 21-34 amino acids and has the core sequence KKPE, which is 26-29 amino acids. KKPE is essential for heparin sulfate attachment. Coiled-coil domain, 43-84 amino acids, contributes to self-oligomerization in vitro. Two cysteines at 71 and 72 create a disulfide bond for the self-assembly of A27, and it interacts with A26. The crystal structure of A27 is a trimeric assembly, consisting of three α-helices, two parallel and one antiparallel. Trimerization can be observed in the N-terminal section. Interaction between trimers occurs at the C-terminal region
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]