Difference between revisions of "Part:BBa K4165052"

 
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This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_J18921), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_J18921), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721).  
 
This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_J18921), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_J18921), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721).  
  
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===Usage and Biology===
 
===Usage and Biology===
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This part performs a multifunction role in our system, the Plug-Sink system. The H1A (BBa_K4165000) peptide directs the whole peptide to bind to the PDZ HtrA1 protein (BBa_K4165004). The second part is the binding peptide which directs the whole peptide-HtrA1 complex to the tau (BBa_K4165009) and amyloid (BBa_K4165005) aggregates. The last part makes the whole system switch on or off based on the presence of the target protein, thus part is the WAP inhibitor (BBa_K4165008).
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4165052 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4165051 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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===Dry-Lab characterization===
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<partinfo>BBa_K4165052 parameters</partinfo>
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===Modelling===
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PDB Structure:
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<p><img src="https://static.igem.wiki/teams/4165/wiki/32.png" style="margin-left:200px;" alt="" width="500" /></p>
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                    Figure 1. The 3D structure of switch 32 modelled by TRrosetta.

Revision as of 14:04, 9 October 2022


HtrA1 Switch 32

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_J18921), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_J18921), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721).


Usage and Biology

This part performs a multifunction role in our system, the Plug-Sink system. The H1A (BBa_K4165000) peptide directs the whole peptide to bind to the PDZ HtrA1 protein (BBa_K4165004). The second part is the binding peptide which directs the whole peptide-HtrA1 complex to the tau (BBa_K4165009) and amyloid (BBa_K4165005) aggregates. The last part makes the whole system switch on or off based on the presence of the target protein, thus part is the WAP inhibitor (BBa_K4165008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 589
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 572
    Illegal AgeI site found at 308
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry-Lab characterization

Modelling

PDB Structure: 


                   Figure 1. The 3D structure of switch 32 modelled by TRrosetta.