Difference between revisions of "Part:BBa K239000"

Revision as of 14:09, 28 September 2009

DegP promoter, activated by Phosporylated CpxR and SigmaE

Sequence contains 3 phosphorylated CpxR (Conjugative Plasmid eXpression) binding sites and transcription is initiated by sigma factor E (24). A native sequence has been used but it has been modified to remove Spe1 restriction site. Function: Can detect various periplasmic stresses (DegP is expressed by the cell to repair damage in the periplasm). Area of application: Hopefully it can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K239000 short</partinfo>
-Sequence contains 3 phosphorylated CpxR binding sites and transcription is initiated by sigma factor E (24). A native sequence has been used but it has been modified to remove Spe1 restriction site.
+Sequence contains 3 phosphorylated CpxR (Conjugative Plasmid eXpression) binding sites and transcription is initiated by sigma factor E (24). A native sequence has been used but it has been modified to remove Spe1 restriction site.
 Function: Can detect various periplasmic stresses (DegP is expressed by the cell to repair damage in the periplasm).
 Area of application: Hopefully it can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress.