Difference between revisions of "Part:BBa K239001"

Revision as of 14:06, 28 September 2009

Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70

Sequence contains 2 phosphorylated BaeR (Bacterial Adaptative rEsponse) binding sites, 1 phosphorylated CpxR (Conjugative Plasmid eXpression) binding site and transcription is initiated by sigma factor 70. Experiments by Bury-Moné et al. (2009) indicates that also RcsB (Regulator of colanic acid Capsule Synthesis) induces expression of the Spy gene. Function: Can detect various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K239001 short</partinfo>
-Sequence contains 2 phosphorylated BaeR binding sites, 1 phosphorylated CpxR binding site and transcription is initiated by sigma factor 70.
+Sequence contains 2 phosphorylated BaeR (Bacterial Adaptative rEsponse) binding sites, 1 phosphorylated CpxR (Conjugative Plasmid eXpression) binding site and transcription is initiated by sigma factor 70. Experiments by Bury-Moné et al. (2009) indicates that also RcsB (Regulator of colanic acid Capsule Synthesis) induces expression of the Spy gene.
-Function: Can detect various periplasmic stresses.
+Function: Can detect various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%).
-Area of application: Hopefully it can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress.
+We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.
 <!-- Add more about the biology of this part here