Difference between revisions of "Part:BBa K4192021"

Revision as of 13:20, 9 October 2022

obcB, one of the genes required for oxalic acid synthesis

It is one of the genes required for oxalic acid synthesis in Burkholderia glumae, which can act together with ObcB to catalyze oxaloacetic acid (OAA) and citric acid to form oxalic acid (OA). When there is only ObcB, it cannot form oxalic acid.

Biology

CAU_China 2022 used this part in their oxalate secretion circuit and the expression product of _obcB_ is one of the main enzyme in in the oxalate secretion circuit^[1]. It can act together with ObcA to catalyze oxaloacetic acid (OAA) and citric acid to form oxalic acid (OA). When there is only ObcB, it cannot form oxalic acid^[2].

Fig.1 Effects of obcA and obcB, the shade of purple reflects the concentration of oxalic acid^[1].

When producing oxalic acid, obcB turns C6-CoA adduct into CoA, acetoacetate,and oxalic acid

Fig.2 Mechanism of obcA and obcB^[2].

Considering the importance of _obcA_ and _obcB_ occur togrther in the biosynthesis of oxalic acid, the CDS of them are in close proximity to each other, even encoded on a single polycistronic message. The implication of this for our project is that we can make these two genes share the same promoter^[2].

Fig.3 Distribution of obcA and obcB^[1].

Sequence and Features

References

[1]Nakata, Paul A, and Cixin He. “Oxalic acid biosynthesis is encoded by an operon in Burkholderia glumae.” FEMS microbiology letters vol. 304,2 (2010): 177-82. doi:10.1111/j.1574-6968.2010.01895.x

[2]Oh, Juntaek et al. “Structural basis for bacterial quorum sensing-mediated oxalogenesis.” The Journal of biological chemistry vol. 289,16 (2014): 11465-11475. doi:10.1074/jbc.M113.543462

Sequence and Features