Difference between revisions of "Part:BBa K4343108"

Latest revision as of 13:07, 9 October 2022

MaElo2

MaElo2 encodes C16/18 elongase from Mortierella alpina which functions in the desaturase and elongase pathway and could convert C16:0 to C18:0.

Description

The wild-type Y. lipolytica Po1f can produce C18:0 under the catalysis of C16/18 elongase. MaElo2 encodes C16/18 elongase of 266 amino acid from Mortierella alpina. Heterologous expression of MaElo2 could catalyze C16:0 to C18:0 in Y. lipolytica.

The enzyme has catalytic activity for C18:1, and according to the experimental results, it produces almost double the percentage of C18:2.

Reference

Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., Xie, D., Short, D. R., ... & Zhu, Q. (2013). Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 845

@@ Line 4: / Line 4: @@
 MaElo2 encodes C16/18 elongase from Mortierella alpina which functions in the desaturase and elongase pathway and could convert C16:0 to C18:0.
+===Description===
+The wild-type Y. lipolytica Po1f can produce C18:0 under the catalysis of C16/18 elongase. MaElo2 encodes C16/18 elongase of 266 amino acid from Mortierella alpina. Heterologous expression of MaElo2 could catalyze C16:0 to C18:0 in Y. lipolytica.
+The enzyme has catalytic activity for C18:1, and according to the experimental results, it produces almost double the percentage of C18:2.
+===Reference===
+Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., Xie, D., Short, D. R., ... & Zhu, Q. (2013). Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740.
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