Difference between revisions of "Part:BBa K4343100"

 
 
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<partinfo>BBa_K4343100 short</partinfo>
 
<partinfo>BBa_K4343100 short</partinfo>
  
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CcDes12 encodes &#8710;-12 desaturase from Coprinus cinereus which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.
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CcDes12 encodes ∆-12 desaturase from Coprinus cinereus which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.
  
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===Description===
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The CcDes12 encodes a protein of 441 amino acids. Heterologous expression of CcDes12 could catalyze C18:1 to C18:2 in Y. lipolytica.
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This enzyme has previously been shown to have high ∆-12 desaturase activity in studies.
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===Reference===
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Zhang, S., Sakuradani, E., Ito, K., & Shimizu, S. (2007). Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD# 822-2. Febs Letters, 581(2), 315-319.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 11:48, 9 October 2022


CcDes12

CcDes12 encodes ∆-12 desaturase from Coprinus cinereus which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.

Description

The CcDes12 encodes a protein of 441 amino acids. Heterologous expression of CcDes12 could catalyze C18:1 to C18:2 in Y. lipolytica. This enzyme has previously been shown to have high ∆-12 desaturase activity in studies.

Reference

Zhang, S., Sakuradani, E., Ito, K., & Shimizu, S. (2007). Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD# 822-2. Febs Letters, 581(2), 315-319. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 95
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 315
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 303
    Illegal BsaI.rc site found at 1062
    Illegal BsaI.rc site found at 1343