Difference between revisions of "Part:BBa K4343112"

 
 
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TsElo5 encodes ∆-5 elongase from Thraustochytrium sp. which functions in the Δ-9 elongase/desaturase pathway and could convert C20:5 to C22:5.
 
TsElo5 encodes ∆-5 elongase from Thraustochytrium sp. which functions in the Δ-9 elongase/desaturase pathway and could convert C20:5 to C22:5.
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===Description===
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The TsELO5 gene e ncodes a ∆-5 elongase of 449 amino acids from Thraustochytrium sp. that catalyzes the conversion of Eicosapentaenoic acid (EPA; C20:5n-3) to Docosapentaenoic acid (DPA; C22:5 n- 3).
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===Literature===
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Hashimoto et al. employed this enzyme in A. limacinum mh0186 to obtain target product yield of 63%, demonstrating the high availability of the enzyme activity.
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===Reference===
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Ohara, J., Sakaguchi, K., Okita, Y., Okino, N., & Ito, M. (2013). Two fatty acid elongases possessing C18-Δ6/C18-Δ9/C20-Δ5 or C16-Δ9 elongase activity in Thraustochytrium sp. ATCC 26185. Marine biotechnology, 15(4), 476-486.
  
 
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Latest revision as of 10:53, 9 October 2022


TsElo5

TsElo5 encodes ∆-5 elongase from Thraustochytrium sp. which functions in the Δ-9 elongase/desaturase pathway and could convert C20:5 to C22:5.

Description

The TsELO5 gene e ncodes a ∆-5 elongase of 449 amino acids from Thraustochytrium sp. that catalyzes the conversion of Eicosapentaenoic acid (EPA; C20:5n-3) to Docosapentaenoic acid (DPA; C22:5 n- 3).

Literature

Hashimoto et al. employed this enzyme in A. limacinum mh0186 to obtain target product yield of 63%, demonstrating the high availability of the enzyme activity.

Reference

Ohara, J., Sakaguchi, K., Okita, Y., Okino, N., & Ito, M. (2013). Two fatty acid elongases possessing C18-Δ6/C18-Δ9/C20-Δ5 or C16-Δ9 elongase activity in Thraustochytrium sp. ATCC 26185. Marine biotechnology, 15(4), 476-486.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 826
    Illegal XhoI site found at 19
    Illegal XhoI site found at 807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 956