Difference between revisions of "Part:BBa K4343099"

Revision as of 10:35, 9 October 2022

FmDes12

FmDes12 encodes ∆-12 desaturase from Fusarium moniliform which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.

Description

The FmDes12 encodes a protein of 477 amino acids. Heterologous expression of FmDes12 could catalyze C18:1 to C18:2 in Y. lipolytica.

Literature

The enzyme has been studied for improving the ratio of ω3 to ω6 fatty acids in microbes and plants.

Reference

Damude, H.G. et al. Identification of bifunctional ∆12/ω3 fatty acid desaturases for improving the ratio of ω3 to ω6 fatty acids in microbes and plants. Proc. Natl. Acad. Sci. USA 103, 9446–9451 (2006).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 683
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 707
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1451

@@ Line 4: / Line 4: @@
 FmDes12 encodes &#8710;-12 desaturase from Fusarium moniliform which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.
+===Description===
+The FmDes12 encodes a protein of 477 amino acids. Heterologous expression of FmDes12 could catalyze C18:1 to C18:2 in Y. lipolytica.
+===Literature===
+The enzyme has been studied for improving the ratio of ω3 to ω6 fatty acids in microbes and plants.
+===Reference===
+Damude, H.G. et al. Identification of bifunctional ∆12/ω3 fatty acid desaturases for improving the ratio of ω3 to ω6 fatty acids in microbes and plants. Proc. Natl. Acad. Sci. USA 103, 9446–9451 (2006).
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