Difference between revisions of "Part:BBa K4488011"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Below is the fluorescence assay depicting a higher reading compared to our previous construct BBa_K4488007 : | Below is the fluorescence assay depicting a higher reading compared to our previous construct BBa_K4488007 : | ||
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| + | [[File:CBD Engineering Cycle.png|frame|Figure 1: Fluorescence readings of fuGFP-linker-CBDs compared to fuGFP-CBDs in different E.coli plasmids. The uninduced and induced plasmids are also compared.]] | ||
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Revision as of 09:37, 9 October 2022
Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end with a linker
The construct can be cloned into an expression vector such as pET28c in E.coli to produce a fusion protein of fuGFP with CBDclos. The fuGFP sequence is towards the N terminus of the protein with CBDclos (BBa_K1321341) downstream followed by a stop codon. Recognition sites for BamHI and BsaI are present before the RBS allowing golden gate cloning. XhoI and BsaI are also present downstream of the stop codon.
Usage and Biology
Below is the fluorescence assay depicting a higher reading compared to our previous construct BBa_K4488007 :
Figure 1: Fluorescence readings of fuGFP-linker-CBDs compared to fuGFP-CBDs in different E.coli plasmids. The uninduced and induced plasmids are also compared.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 163
- 1000COMPATIBLE WITH RFC[1000]