Difference between revisions of "Part:BBa K4156098"

Revision as of 08:48, 9 October 2022

pCadC-Bxb1-φ174E

pCadC-Bxb1-φ174E is a complex component expressing the cleavage gene φ174E, constructed from the pH-sensitive promoter pCadC and the serine integrase Bxb1.

Usage and Biology

We designed pCadC-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate coupling the pCadC promoter to Bxb1. We will validate the function of this biobrick by measuring bacterial viability.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 368
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 642
Illegal XhoI site found at 729
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1476

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4156098 short</partinfo>
-pCadC-Bxb1is constructed with pH-sensitive promoter pCadC and serine integrase Bxb1.
+pCadC-Bxb1-φ174E is a complex component expressing the cleavage gene φ174E, constructed from the pH-sensitive promoter pCadC and the serine integrase Bxb1.
 <!-- Add more about the biology of this part here -->
@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-pCadC is regulated by the membrane lineage activator protein (CadC) and exhibits higher activity in acidic medium than in neutral pH medium. It is used in response to acidic conditions to allow the strain to rapidly localize to tumor cells and activate downstream gene transcription.Bxb1 is used to control downstream logic gate switching and to provide gene signaling amplification.
+We designed pCadC-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate coupling the pCadC promoter to Bxb1. We will validate the function of this biobrick by measuring bacterial viability.
 <span class='h3bb'>Sequence and Features</span>