Difference between revisions of "Part:BBa K4192130"

 
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<partinfo>BBa_K4192130 short</partinfo>
 
<partinfo>BBa_K4192130 short</partinfo>
  
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The gene yf1, fixJ and PfixK2 make up the system of blue light regulation. Under dark conditions, phosphate groups are transferred from Yf1 protein to FixJ protein, and the phosphorylated FixJ protein activates the PfixK2 promoter, which in turn activates downstream gene expression. Under light induction, the phosphorylation of Fixj protein was blocked and the expression of genes regulated by the PfixK2 promoter was suppressed.The mCherry protein emits red fluorescence to characterize the efficiency of control elements in regulating gene expression.
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The gene <i>yf1</i>, <i>fixJ</i> and P<i>fixK2</i> make up the system of blue light regulation. Under dark conditions, phosphate groups are transferred from Yf1 protein to FixJ protein, and the phosphorylated FixJ protein activates the P<i>fixK2</i> promoter, which in turn activates downstream gene expression. Under light induction, the phosphorylation of Fixj protein was blocked and the expression of genes regulated by the P<i>fixK2</i> promoter was suppressed.The mCherry protein emits red fluorescence to characterize the efficiency of control elements in regulating gene expression.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:47, 9 October 2022


Plac-RBS-yf1-RBS-fixJ-Pfixk2-RBS-mcherry

The gene yf1, fixJ and PfixK2 make up the system of blue light regulation. Under dark conditions, phosphate groups are transferred from Yf1 protein to FixJ protein, and the phosphorylated FixJ protein activates the PfixK2 promoter, which in turn activates downstream gene expression. Under light induction, the phosphorylation of Fixj protein was blocked and the expression of genes regulated by the PfixK2 promoter was suppressed.The mCherry protein emits red fluorescence to characterize the efficiency of control elements in regulating gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 210
    Illegal SpeI site found at 1361
    Illegal SpeI site found at 2257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 210
    Illegal SpeI site found at 1361
    Illegal SpeI site found at 2257
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 210
    Illegal SpeI site found at 1361
    Illegal SpeI site found at 2257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 210
    Illegal SpeI site found at 1361
    Illegal SpeI site found at 2257
    Illegal NgoMIV site found at 764
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 926
    Illegal NgoMIV site found at 944
    Illegal NgoMIV site found at 1450
    Illegal NgoMIV site found at 1743
    Illegal NgoMIV site found at 1837
    Illegal AgeI site found at 478
    Illegal AgeI site found at 1618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1507
    Illegal BsaI.rc site found at 377