Difference between revisions of "Part:BBa K4156077"

 
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<partinfo>BBa_K4156077 short</partinfo>
 
<partinfo>BBa_K4156077 short</partinfo>
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Hyper-spank is an IPTG inducible promoter, designed to be part of Llprd operon
  
Promoter hyper-spank is an IPTG inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl &#946;-D-1-thiogalactopyranoside (IPTG).
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===Usage and Biology===
 
===Usage and Biology===
  
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Hyper-spank promoter is an IPTG inducible promoter that has been designed for high expression. Gene expression under the control of the hyper-spank promoter can be induced by addition of IPTG. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR operon subunit (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_BBa_K4156101"> BBa_BBa_K4156101 </a></html>).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156077 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156077 SequenceAndFeatures</partinfo>

Revision as of 08:24, 9 October 2022


Promoter Hyper-spank Hyper-spank is an IPTG inducible promoter, designed to be part of Llprd operon

Usage and Biology

Hyper-spank promoter is an IPTG inducible promoter that has been designed for high expression. Gene expression under the control of the hyper-spank promoter can be induced by addition of IPTG. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR operon subunit ( BBa_BBa_K4156101 ).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]