Difference between revisions of "Part:BBa K4156076:Design"
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domain of a transmembrane transcriptional activator. Sci Rep. Apr 21 2017;7(1):1051.  
 
domain of a transmembrane transcriptional activator. Sci Rep. Apr 21 2017;7(1):1051.  
 
doi:10.1038/s41598-017-01031-9  
 
doi:10.1038/s41598-017-01031-9  
 +
 +
 
2 Viala JP, Méresse S, Pocachard B, Guilhon AA, Aussel L, Barras F. Sensing and adaptation to  
 
2 Viala JP, Méresse S, Pocachard B, Guilhon AA, Aussel L, Barras F. Sensing and adaptation to  
 
low pH mediated by inducible amino acid decarboxylases in Salmonella. PLoS One.  
 
low pH mediated by inducible amino acid decarboxylases in Salmonella. PLoS One.  
 
2011;6(7):e22397. doi:10.1371/journal.pone.0022397
 
2011;6(7):e22397. doi:10.1371/journal.pone.0022397
 +
 +
 
3 Lee YH, Kim JH, Bang IS, Park YK. The membrane-bound transcriptional regulator CadC is  
 
3 Lee YH, Kim JH, Bang IS, Park YK. The membrane-bound transcriptional regulator CadC is  
 
activated by proteolytic cleavage in response to acid stress. J Bacteriol. Jul 2008;190(14):5120-6.  
 
activated by proteolytic cleavage in response to acid stress. J Bacteriol. Jul 2008;190(14):5120-6.  
 
doi:10.1128/jb.00012-08
 
doi:10.1128/jb.00012-08
 
</i>
 
</i>

Revision as of 08:23, 9 October 2022


Promoter pCadC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 358


Design Notes

pCadC is regulated by membrane-tethered activator protein (CadC), exhibits higher activity in acidic media than in media at neutral pH. In pH reporter strains, it’s used to test their response to acidic conditions in tumors induction.


Source

Escherichia coli

References

1 Schlundt A, Buchner S, Janowski R, et al. Structure-function analysis of the DNA-binding domain of a transmembrane transcriptional activator. Sci Rep. Apr 21 2017;7(1):1051. doi:10.1038/s41598-017-01031-9


2 Viala JP, Méresse S, Pocachard B, Guilhon AA, Aussel L, Barras F. Sensing and adaptation to low pH mediated by inducible amino acid decarboxylases in Salmonella. PLoS One. 2011;6(7):e22397. doi:10.1371/journal.pone.0022397


3 Lee YH, Kim JH, Bang IS, Park YK. The membrane-bound transcriptional regulator CadC is activated by proteolytic cleavage in response to acid stress. J Bacteriol. Jul 2008;190(14):5120-6. doi:10.1128/jb.00012-08