Difference between revisions of "Part:BBa K4432020"

Latest revision as of 08:08, 9 October 2022

CrtW: β−carotene ketolase

This part is an Escherichia coli codon optimized version of the ß-carotene-ketolase (CrtW148) from Nostoc punctiforme PCC 73102 (UniProt B2IZ40).

Usage and Biology

The ß-carotene-ketolase (EC 1.14.99.63) catalyses the conversion of ß-carotene into echinenone and of echinenone into canthaxanthin (Figure 1).

Figure 1. Conversion of ß-carotene into canthaxanthin by EC: 1.14.99.63.

The Nostoc punctiforme CrtW [1] is a 30.743 kDa protein of 262 amino acids.

When placed under the control of the hybrid T5 promoter regulated by LacI (BBa_K4432000) or the classical pLac promoter (BBa_R0010), this part leads to the synthesis of canthaxanthin in an E. coli producing ß-carotene.

For further details, see the Main Pages on the Registry of the CrtW expression plasmids (BBa_K4432120, BBa_K4432220 and BBa_K4432320).

References

[1] Steiger S, Sandmann G. Cloning of two carotenoid ketolase genes from Nostoc punctiforme for the heterologous production of canthaxanthin and astaxanthin. Biotechnology Letters (2004) 26: 813–817.

Sequence and Features