Difference between revisions of "Part:BBa K4414003"

 
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<partinfo>BBa_K4414003 short</partinfo>
 
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Nuclear export signal
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This nuclear export signal（NES）directs the protein out of the nucleus.
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<img src="https://static.igem.wiki/teams/4414/wiki/03-1.png" class="figure-img img-fluid rounded"  height="200px">
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==Usage and Biology==
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Regulating the output of many proteins from the nucleus depends on the presence of a nuclear export signal(NES) consisting of leucine-rich amino acids.(Fornerod et al., 1997);(Fischer et al., 1995). The shuttle receptor that appears to bind to the NES sequence and function in the protein output from the nucleus to the cytoplasm is CRM1 (chromosomal region maintenance)/export protein 1.(Richards et al., 1997) CRM1 binds to Ran GTPase and interacts with the nucleoporous components to translocate NES-containing proteins to the cytoplasm.(Stade et al., 1997)
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===Sequecing===
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The plasmid was sequenced correct.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4414003 SequenceAndFeatures</partinfo>
 
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<partinfo>BBa_K4414003 parameters</partinfo>
 
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===Functional characterization===
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We can connect NES to EGFP and observe the location of fluorescent proteins to verify the function of NES
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<figure class="figure">
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<img src="https://static.igem.wiki/teams/4414/wiki/031-2.png" class="figure-img img-fluid rounded"  height="200px">
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<img src="https://static.igem.wiki/teams/4414/wiki/031-3.png" class="figure-img img-fluid rounded"  height="200px">
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<img src="https://static.igem.wiki/teams/4414/wiki/031-4.png" class="figure-img img-fluid rounded"  height="200px">
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</figure>
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===Reference===
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[1].Fischer, U., Huber, J., Boelens, W. C., Mattaj, I. W., & Lührmann, R. (1995). The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell, 82(3), 475–483. https://doi.org/10.1016/0092-8674(95)90436-0
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[2].Fornerod, M., Ohno, M., Yoshida, M., & Mattaj, I. W. (1997). CRM1 is an export receptor for leucine-rich nuclear export signals. Cell, 90(6), 1051–1060. https://doi.org/10.1016/s0092-8674(00)80371-2
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[3].Richards, S. A., Carey, K. L., & Macara, I. G. (1997). Requirement of guanosine triphosphate-bound ran for signal-mediated nuclear protein export. Science (New York, N.Y.), 276(5320), 1842–1844. https://doi.org/10.1126/science.276.5320.1842

Revision as of 07:48, 9 October 2022


NES

This nuclear export signal（NES）directs the protein out of the nucleus.

Usage and Biology

Regulating the output of many proteins from the nucleus depends on the presence of a nuclear export signal(NES) consisting of leucine-rich amino acids.(Fornerod et al., 1997);(Fischer et al., 1995). The shuttle receptor that appears to bind to the NES sequence and function in the protein output from the nucleus to the cytoplasm is CRM1 (chromosomal region maintenance)/export protein 1.(Richards et al., 1997) CRM1 binds to Ran GTPase and interacts with the nucleoporous components to translocate NES-containing proteins to the cytoplasm.(Stade et al., 1997)


Sequecing

The plasmid was sequenced correct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional characterization

We can connect NES to EGFP and observe the location of fluorescent proteins to verify the function of NES


Reference

[1].Fischer, U., Huber, J., Boelens, W. C., Mattaj, I. W., & Lührmann, R. (1995). The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell, 82(3), 475–483. https://doi.org/10.1016/0092-8674(95)90436-0 [2].Fornerod, M., Ohno, M., Yoshida, M., & Mattaj, I. W. (1997). CRM1 is an export receptor for leucine-rich nuclear export signals. Cell, 90(6), 1051–1060. https://doi.org/10.1016/s0092-8674(00)80371-2 [3].Richards, S. A., Carey, K. L., & Macara, I. G. (1997). Requirement of guanosine triphosphate-bound ran for signal-mediated nuclear protein export. Science (New York, N.Y.), 276(5320), 1842–1844. https://doi.org/10.1126/science.276.5320.1842