Difference between revisions of "Part:BBa K4192141"
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===Usage=== | ===Usage=== | ||
<p>As an indispensable part of the light control system, Yf1 affects the phosphorylation of FixL and then regulates the transcription of downstream genes by regulating the activity of the promoter.The YF1-containing light control system was introduced into the engineered bacteria to make them light-responsive and could be manipulated by light in a spatially and temporally accurate, reversible and non-harmful way.However,the N37C mutation of <i>yf1</i> has a better effect on regulation, that is, more target genes can be expressed under light conditions, and leakage expression can be minimized under light conditions.</p> | <p>As an indispensable part of the light control system, Yf1 affects the phosphorylation of FixL and then regulates the transcription of downstream genes by regulating the activity of the promoter.The YF1-containing light control system was introduced into the engineered bacteria to make them light-responsive and could be manipulated by light in a spatially and temporally accurate, reversible and non-harmful way.However,the N37C mutation of <i>yf1</i> has a better effect on regulation, that is, more target genes can be expressed under light conditions, and leakage expression can be minimized under light conditions.</p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 07:34, 9 October 2022
yf1(N37C)
The gene yf1 encodes a fusion kinase.The kinase yf1 ecxpressed controls gene expression in a light-dependent manner in vivo.The yf1 N37C can induce the expression of more downstream genes in the dark environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 616
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 724
Illegal AgeI site found at 258 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 157