Difference between revisions of "Part:BBa K4192120"

 
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<partinfo>BBa_K4192120 short</partinfo>
 
<partinfo>BBa_K4192120 short</partinfo>
  
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The expression products of obcA and obcB are enzymes needed to produce oxalate from citric acid and acetyl-CoA. And FpOAR protein is the oxalate efflux channel. The composite part of these three genes can efflux oxalic acid out of the thallus.  
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The expression products of <i>obcA</i> and <i>obcB</i> are enzymes needed to produce oxalate from citric acid and acetyl-CoA. And FpOAR protein can efflux oxalate. The composite part of these three genes can efflux oxalic acid out of the thallus.  
 
Because it only have one promoter at the beginning of the whole genetic circuit, the efflux rate of the generated oxalic acid is relatively low, which may also lead to the death of the bacteria more easily due to excessive accumulation of oxalic acid in the body.
 
Because it only have one promoter at the beginning of the whole genetic circuit, the efflux rate of the generated oxalic acid is relatively low, which may also lead to the death of the bacteria more easily due to excessive accumulation of oxalic acid in the body.
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We have already tested it in E.coli and Pseudomonas fluorescens.
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We have already tested it in <i>E.coli</i> and <i>Pseudomonas fluorescens</i>.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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<p>This part is originated from <i>Burkholderia glumae</i> and <i>Fomitopsis palustris</i>. In our project we used this part in pBBR1MCS2 plasmid to run the tests. We tested.his part by the pH changes in the bacterial solution and by oxalic acid content detection kit (BoxBio, Beijing) to demonstrate it can produce and secrete oxalic acid in <i>E. coli</i> and <i>Pseudomonas fluorescens</i>.
  
 
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Revision as of 07:32, 9 October 2022


Plac-obcA-obcB-Fpoar, Oxalate secretion gene circuit

The expression products of obcA and obcB are enzymes needed to produce oxalate from citric acid and acetyl-CoA. And FpOAR protein can efflux oxalate. The composite part of these three genes can efflux oxalic acid out of the thallus. Because it only have one promoter at the beginning of the whole genetic circuit, the efflux rate of the generated oxalic acid is relatively low, which may also lead to the death of the bacteria more easily due to excessive accumulation of oxalic acid in the body. We have already tested it in E.coli and Pseudomonas fluorescens.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2552
    Illegal PstI site found at 397
    Illegal PstI site found at 2695
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2552
    Illegal PstI site found at 397
    Illegal PstI site found at 2695
    Illegal NotI site found at 2330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2552
    Illegal BglII site found at 1541
    Illegal BglII site found at 3555
    Illegal XhoI site found at 304
    Illegal XhoI site found at 1428
    Illegal XhoI site found at 3367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2552
    Illegal PstI site found at 397
    Illegal PstI site found at 2695
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2552
    Illegal PstI site found at 397
    Illegal PstI site found at 2695
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 720
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 784
    Illegal NgoMIV site found at 1014
    Illegal NgoMIV site found at 1791
    Illegal NgoMIV site found at 2208
    Illegal AgeI site found at 326
  • 1000
    COMPATIBLE WITH RFC[1000]