Difference between revisions of "Part:BBa K4414001"

 
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A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins into the nucleus. NLS motifs play a key role in this mechanism; 1. typically, deletion of the NLS disrupts nuclear import; and 2. frequently, a non-nuclear protein will be imported into the nucleus if fused to an NLS.
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This nuclear localization sequence（NLS） derived from SV40 directs the protein into the nucleus.
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<img src="https://static.igem.wiki/teams/4414/wiki/01-1.png" class="figure-img img-fluid rounded"  height="200px">
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==Usage and Biology==
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A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus.(Cokol et al., 2000)
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===Sequecing===
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The plasmid was sequenced correct.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4414001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4414001 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K4414001 parameters</partinfo>
 
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===Functional characterization ===
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We can connect NLS to EGFP and observe the location of fluorescent proteins to verify the function of NLS.
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===Reference===
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[1].Weikum ER, Knuesel MT, Ortlund EA, Yamamoto KR. Glucocorticoid receptor control of transcription: precision and plasticity via allostery. Nat Rev Mol Cell Biol. 2017 Mar;18(3):159-174. doi: 10.1038/nrm.2016.152. Epub 2017 Jan 5. PMID: 28053348; PMCID: PMC6257982.

Revision as of 07:28, 9 October 2022


nuclear localization signal（NLS）

This nuclear localization sequence（NLS） derived from SV40 directs the protein into the nucleus.

Usage and Biology

A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus.(Cokol et al., 2000)

Sequecing

The plasmid was sequenced correct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional characterization

We can connect NLS to EGFP and observe the location of fluorescent proteins to verify the function of NLS.

Reference

[1].Weikum ER, Knuesel MT, Ortlund EA, Yamamoto KR. Glucocorticoid receptor control of transcription: precision and plasticity via allostery. Nat Rev Mol Cell Biol. 2017 Mar;18(3):159-174. doi: 10.1038/nrm.2016.152. Epub 2017 Jan 5. PMID: 28053348; PMCID: PMC6257982.